Research Paper Volume 12, Issue 6 pp 5091—5120

Time-dependent interactions of blood platelets and cancer cells, accompanied by extramedullary hematopoiesis, lead to increased platelet activation and reactivity in a mouse orthotopic model of breast cancer – implications for pulmonary and liver metastasis

Figure 4. Representative histopathological images of the extramedullary hematopoiesis foci in spleen of mice injected with 4T1 cancer cells. Extramedullary hematopoiesis was diagnosed histologically at time interval t5 in slides from spleens isolated during necropsy in mice injected with saline (control) (A, C, E) or 4T1 cancer cells (B, D, F). Hematoxylin and eosin staining, magnification of 20X (A, B), 100X (C, D) and 600X (E, F). Extramedullary hematopoiesis foci are marked with white arrows. More experimental details are given in the Materials and methods section.