Research Paper Volume 12, Issue 6 pp 5152—5167

Rapamycin protects chondrocytes against IL-18-induced apoptosis and ameliorates rat osteoarthritis

Figure 2. IL-18 stimulation increased the protein levels of pro-apoptosis genes and decreased the protein levels of anti-apoptosis genes. Western blot (A) measuring protein levels of Bax (B), Bcl2 (C), Cleaved Caspase3 (D), Caspase3 (E), Cleaved Caspase9 (F), Caspase9 (G), and GAPDH as an internal control in total extract from chondrocytes treated with IL-18 at different concentrations for 24 h. The values are expressed as mean ± standard deviation (SD). Significance was calculated by a one-way ANOVA with a post hoc Tukey's multiple comparisons test. *p<0.05 versus 0ng/ml IL-18 treated group.