Figure 3. IL-18 stimulation induced autophagy deficiency of chondrocytes in vitro. Protein levels of Atg5 (B), Atg7 (C), Beclin1 (D), LC3B/LC3A (E), P62 (F), and GAPDH as an internal control in chondrocytes incubated without or with IL-18 (100 ng/ml) for various durations as evaluated by Western blot (A). The values are expressed as mean ± standard deviation (SD). Significance was calculated by a one-way ANOVA with a post hoc Tukey's multiple comparisons test. *p<0.05 versus without IL-18 treated group.