Research Paper Volume 12, Issue 6 pp 5152—5167

Rapamycin protects chondrocytes against IL-18-induced apoptosis and ameliorates rat osteoarthritis

Figure 6. IL-18 stimulation induced autophagy deficiency via PI3K/Akt/mTOR signaling pathway. The chondrocytes were treated with IL-18 at different concentrations for 24 h. Protein levels of p-PI3K (B), PI3K (C), p-Akt (D), Akt (E), p-mTOR (F), mTOR (G), and GAPDH as an internal control in total extract, analyzed by Western blot (A). The values are expressed as mean ± standard deviation (SD). Significance was calculated by a one-way ANOVA with a post hoc Tukey's multiple comparisons test. *p<0.05 versus 0 ng/ml IL-18 treated group.