Figure 12. Validation of hsa_circ_0000977-mediated miRNA-mRNA sub-network in CRC using clinical tissue samples. Expression of hsa_circ_0000977 (A), hsa-miR-135b-5p (B), FOXO1 (C), MBNL1 (D), MEF2C (E), RECK (F), PPM1E (G), TTLL7 (H) and PCP4L1 (I) in CRC cancer samples compared with normal samples. Expression correlation between hsa_circ_0000977/ hsa-miR-135b-5p (J), hsa-miR-135b-5p/FOXO1 (K), hsa-miR-135b-5p/MBNL1 (L), hsa-miR-135b-5p/MEF2C (M), hsa-miR-135b-5p/RECK (N), hsa-miR-135b-5p/PPM1E (O), hsa-miR-135b-5p/TTLL7 (P), hsa-miR-135b-5p/PCP4L1 (Q), hsa_circ_0000977/FOXO1 ®, hsa_circ_0000977/MBNL1 (S), hsa_circ_0000977/MEF2C (T), hsa_circ_0000977/RECK (U), hsa_circ_0000977/PPM1E (V), hsa_circ_0000977/TTLL7 (W) and hsa_circ_0000977/PCP4L1 (X) pairs in CRC. *p<0.05 represents significant difference.