Research Paper Volume 12, Issue 6 pp 5280—5299

m6A-induced lncRNA MALAT1 aggravates renal fibrogenesis in obstructive nephropathy through the miR-145/FAK pathway

Figure 6. MALAT1 regulates the MALAT1/miR-145/FAK axis in HK2 cells treated with TGF-β1. (A and B) qRT-PCR and western blot analyses of FAK expression in HK2 cells treated with TGF-β1 alone or together with siMALAT1, a miR-145 inhibitor or a NC. (C) Western blot analyses of E-cadherin, vimentin, α-SMA and collagen I expression in HK2 cells receiving different treatments. (D and E) CCK8, EdU and cell migration analyses of the viability, proliferation and migration of HK2 cells receiving different treatments. GAPDH was used as a control. *P < 0.05 and **P < 0.01.