Figure 4. Verapamil extends lifespan by inhibiting the activity of calcineurin. (A) Verapamil (100 μM) reduced the activity of calcineurin in C. elegans (***P < 0.001). Cyclosporin A (CsA) was used as positive control (***P < 0.001). An unpaired t-test was used to calculate the P-values and error bars represent SEM. (B) Verapamil (100 μM) did not extend the lifespan of worms treated with tax-6 RNAi. The log-rank (Mantel-Cox) test was used to assess the P-values.