Research Paper Volume 12, Issue 6 pp 5352—5361

Metformin inhibits high glucose-induced smooth muscle cell proliferation and migration

Figure 4. miR-142-3p overexpression by mimic and miR-142-3p inhibition by siRNA resulted in inhibition and promotion, respectively, of high glucose–induced vascular smooth muscle cell (VSMC) migration enhancement via the HMGB1-autophagy related pathway, whereas metformin abolished the effects of miR-142-3p siRNA. (A) Scratch assay for the effects of miR-142-3p overexpression and inhibition. (B) Transwell assay for the effects of miR-142-3p overexpression and inhibition. (C) miR-142-3p overexpression by mimic results in decreased HMGB1 and LC3II and elevated p62 level in high glucose–induced VSMC migration enhancement, whereas miR-142-3p siRNA caused the opposite effects and metformin abolished the effects of miR-142-3p siRNA. (D) Schematic of the role of metformin in the regulation of VSMC proliferation and migration. *p < 0.05, **p < 0.01 and ***p < 0.001 for between-group comparison.