Research Paper Volume 12, Issue 7 pp 5764—5780

CK1δ as a potential therapeutic target to treat bladder cancer

Figure 3. Compound 13i HCl induces apoptosis in RT112 cells. (A) RT112 cells were exposed to the indicated concentrations of 13i HCl for 72 h and subjected to cell cycle analysis. Data are represented as mean ± S.D. *P<0.05, **P<0.01, ***P<0.001 compared to control cells (n=3). (B) RT112 cells were treated with 13i HCl (10 μM) for the indicated times and cell cycle distribution was measured by PI staining and flow cytometry (n=2). (C, D) RT112 cells were exposed to the indicated concentrations of 13i HCl for 72 h (C) or 13i HCl (10 μM) for different times (D) and subjected to Western blotting with the indicated antibodies.