Figure 3. Compound 13i HCl induces apoptosis in RT112 cells. (A) RT112 cells were exposed to the indicated concentrations of 13i HCl for 72 h and subjected to cell cycle analysis. Data are represented as mean ± S.D. *P<0.05, **P<0.01, ***P<0.001 compared to control cells (n=3). (B) RT112 cells were treated with 13i HCl (10 μM) for the indicated times and cell cycle distribution was measured by PI staining and flow cytometry (n=2). (C, D) RT112 cells were exposed to the indicated concentrations of 13i HCl for 72 h (C) or 13i HCl (10 μM) for different times (D) and subjected to Western blotting with the indicated antibodies.