Figure 6. Effects of 13i HCl on migratory activity of bladder cancer cells. (A) RT112 cells were seeded into a 2-well insert on 6-well plate and allowed to attach overnight. The wound was created by removing the insert, and the cells were treated with or without 13i HCl and the images were captured at indicated times by EVOS XL Core Cell Imaging System (Thermo Scientific). Scale bar = 100 μm. (B) Quantification of wound healing assay. Data are represented as mean ± S.D. (n=2) *P<0.05 compared to control cells. (C) RT112 cells were exposed to indicated concentrations of 13i HCl for 72 h and subjected to Western blotting for the detection of EMT markers.