Research Paper Volume 12, Issue 7 pp 5781—5791

A novel germline gain-of-function HIF2A mutation in hepatocellular carcinoma with polycythemia

Figure 4. Transcription of the mutant HIF-2α protein is maintained under normoxic and hypoxic conditions. (A) Results from real-time PCR demonstrated similar transcription levels for the wildtype and mutant HIF2A genes under conditions of normoxia and simulated hypoxia. (B) A luciferase assay using Hep3B cells transfected with wildtype and p.E549K HIF2A confirmed these findings.