Research Paper Volume 12, Issue 6 pp 5516—5538

Microarray analysis of verbenalin-treated human amniotic epithelial cells reveals therapeutic potential for Alzheimer’s Disease

Figure 6. Effect of verbenalin treatment on the expressions of EGF, VEGF, and NRG1 in Aβ-induced human neuroblastoma SH-SY5Y cells. (A) Gene expressions were evaluated by real-time PCR. Each value represents the mean ± SD (n = 4). (B) Boxplots of protein concentration (ng/ml) obtained by ELISA (n = 4). Box ranges from 25th to 75th percentile; the line in the middle represents the median value; the error bar represents the SD. Asterisks refer to statistical significance (*p < 0.05, **p < 0.01, ***p < 0.001) by One-way ANOVA followed by Dunnett’s multiple comparisons test (for linear distribution) as compared with only Aβ-treated group.