Research Paper Volume 12, Issue 10 pp 8837—8857

The TβR II-targeted aptamer S58 prevents fibrosis after glaucoma filtration surgery

Figure 2. S58 treatment prolongs the survival of filtering blebs after GFS in rabbits. (A) Animal model establishment and postoperative model of administration. (B) IOPs of the operated and non-operated eyes were measured. The numbers indicated the mean IOP percentage of the control group (n=5). (C) Representative stereo-microscopic images showing bleb characteristics in different groups of rabbits’ eyes (the area of blebs is outlined by dotted blue lines) at day 3, 7, and 14 after GFS, and quantization of bleb area. (D) UBM imaging of the rabbits’ conjunctiva confirmed bleb survival. (E) Representative images of H&E (shown by black arrow), Masson’s trichrome staining (as white arrow showed) and Sirius Red staining (n=5). (F) Representative images showing immunohistochemical staining for α-SMA, vimentin, and collagen-1 in rabbits’ ocular tissue sections, collected at day 14 after GFS. (Nuclei = blue, vimentin = red, and α-SMA/ collagen-1= green). (G) Fibrotic protein levels of α-SMA/collagen-1 was examined by western blotting. GAPDH was used as loading control. n = 3. Data indicate the mean ± SD. *p < 0.05, **p < 0.001.