Research Paper Volume 12, Issue 10 pp 8837—8857

The TβR II-targeted aptamer S58 prevents fibrosis after glaucoma filtration surgery

Figure 6. S58 reduces TGF-β2-induced HConFs fibrosis. (A) Effect of TGF-β2 and S58 on HConFs viability (B) Western blot of fibrosis-related proteins. (C) Representative images and quantification of cell motility of TGF-β2-treated HConFs with or without the presence of S58 at specified times (Dotted blue lines: edges of the migrated cells). (D) mRNA levels of fibronectin, collagen-1, α-SMA and collagen-3a. (EG) Levels of α-SMA, fibronectin, and collagen-1 were analyzed by immunofluorescence staining after 24h treatment (Nuclei = blue, α-SMA = green, fibronectin/collagen-1 = red). Data indicate the mean ± SD. n=3. *p < 0.05, **p < 0.01, ***p < 0.001.