Research Paper Volume 12, Issue 10 pp 8837—8857

The TβR II-targeted aptamer S58 prevents fibrosis after glaucoma filtration surgery

Figure 7. S58 promotes antioxidant defense against TGF-β2-induced fibrosis in HConFs via the activation of PI3K/Akt/Nrf2 signaling pathway. (A) p-Nrf2(S40), Nrf2, Akt, p-Akt (308) and p-Akt (473) levels in cell lysates from TGF-β2-pretreated HConFs for 24h. (B) Screening for specific siRNAs to knock down Nrf2 protein. (C, E) Relative levels of fibrotic proteins and (D, F) antioxidant defense proteins in whole cell lysates from S58-treated HConFs with/without siRNA-Nrf2 transfection(or LY294002 (40×10-6 m) after 72h. (G) Fibrosis levels were analyzed by co-staining of α-SMA and collagen-1 immunofluorescence.