Figure 1. GSG2 was up-regulated in bladder cancer. (A) The expression of GSG2 in bladder cancer tissues and normal tissues was detected by IHC. (B) Data mining of TCGA database showed that expression of GSG2 is relatively higher in bladder cancer tissues compared with normal tissues. (C) Endogenous expression of GSG2 in human bladder epithelial cell line HCV29 and bladder cancer cell lines including RT4, EJ, T24 and J82 was detected by qPCR. (D) Kaplan-Meier survival analysis was performed to reveal the relationship between GSG2 expression and prognosis of bladder cancer patients. The figures are representative data from at least three independent experiments. The data were expressed as mean ± SD (n ≥ 3), *P<0.05, **P<0.01, ***P<0.001.