Figure 4. KIF15 was identified as the downstream of GSG2 by RNA-sequencing. (A) RNA-sequencing (3 v 3) was performed to identify differentially expressed genes in T24 cells with or without GSG2 knockdown. (B, C) The mRNA and protein levels of several top significant DEGs in shGSG2 group identified by RNA sequencing further detected in T24 cells were detected by qPCR (B) and western blotting (C), respectively. (D) The IPA analysis was performed to produce the GSG2-related interaction network. (E) The data mining of TCGA database showed the upregulation of KIF15 expression in bladder cancer tissues. (F) The expression of KIF15 in bladder cancer and normal tissues was detected by IHC. (G) The direct interaction between GSG2 and KIF15 was proved by co-immunoprecipitation. The data were expressed as mean ± SD (n ≥ 3), *P<0.05, **P<0.01, ***P<0.001.