Research Paper Volume 12, Issue 10 pp 8858—8879

GSG2 (Haspin) promotes development and progression of bladder cancer through targeting KIF15 (Kinase-12)

Figure 5. Overexpression of GSG2 promoted bladder cancer and knockdown of KIF15 inhibited bladder cancer. (A) The efficiency of GSG2 overexpression in T24 cells was evaluated by qPCR. (B) The successful overexpression of GSG2 in T24 cells was confirmed by western blotting. (C) Celigo cell counting assay was performed to examine the effects of GSG2 overexpression on cell proliferation of T24 cells. (D) The ability of T24 cells with or without GSG2 overexpression to form colonies was examined by colony formation assay. (E) Flow cytometry was performed to detect cell apoptosis of T24 cells with or without GSG2 overexpression. (F, G) The effects of GSG2 overexpression on cell migration of T24 cells were evaluated by wound-healing (F) and Transwell (G) assays. (H) The efficiency of KIF15 knockdown in T24 cells was evaluated by qPCR. (I) The successful knockdown of KIF15 in T24 cells was confirmed by western blotting. (J) Celigo cell counting assay was performed to examine the effects of KIF15 knockdown on cell proliferation of T24 cells. (K) The ability of T24 cells with or without KIF15 knockdown to form colonies was examined by colony formation assay. (L) Flow cytometry was performed to detect cell apoptosis of T24 cells with or without KIF15 knockdown. (M, N) The effects of KIF15 knockdown on cell migration of T24 cells were evaluated by wound-healing (M) and Transwell (N) assays. The figures are representative data from at least three independent experiments. The data were expressed as mean ± SD (n ≥ 3), *P<0.05, **P<0.01, ***P<0.001.