Figure 1. OGFRP1 was upregulated in PCa and located in the cytoplasm. (A) OGFRP1 levels in 57 pairs of PCa and adjacent tissues were detected by qPCR. (B) OGFRP1 was upregulated in PCa cell lines (PC-3, DU-145, C4-2 and VCAP) compared with a normal human prostatic epithelial cell line (RWPE-1). (C) Subcellular location of OGFRP1 in DU-145 and PC-3 cells was analyzed using FISH. (D and E) qPCR demonstrated that siOGFRP1 transfection inhibits mRNA expression of OGFRP1 in both DU-145 and PC-3 cells. SiRNA2 for DU145 and siRNA3 for PC3 were applied in all of the following experiments due to the interference efficiency. All experiments were performed 3 times. *P<0.05; **P<0.01.