Figure 2. OGFRP1 decoyed miR-124-3p in PCa cells. (A) Luciferase assay was performed to detect the binding of OGFRP1 to miRNAs (miR299-3p, miR-224-5p, miR-124-3p, miR-134-5p, and miR-624a-5p). (B) Luciferase reporter gene assay was performed to confirm the binding of OGFRP1 to miR-124-3p. (C) qPCR results proved that OGFRP1 knockdown lead to the up-regulation of miR-124-3p levels. (D and E) The association of OGFRP1 and miR-124-3p between Ago2 in DU-145 and PC-3 cells was confirmed using RIP. *P<0.05; **P<0.01.