Figure 6. MiR-124-3p inhibited the expression of SARM1 via binding to its 3’ UTR. (A) The mRNA levels of the predicted target genes (MYLIP, SARM1, NARF, CSDE1, and NEDD4) in PCa cells transfected with miR-124-3p mimics were detected using qPCR. (B and C) Luciferase reporter gene assay was performed to confirm the binding of miR-124-3p to SARM1. (D) The expression of SARM1 was detected by western blot in DU-145 and PC-3 cells. (E and F) miR-124-3p and SARM1 levels in 57 pairs of PCa and adjacent tissues were detected by qPCR. (G–I) The correlation between OGFRP1, miR-124-3p, and SARM1 levels was analyzed. *P<0.05; **P<0.01; ***P<0.001.