Research Paper Volume 12, Issue 10 pp 8893—8912

Figure 7. The biological role of lnc-LOC645166 in breast cancer cells was mediated by NF-κB and GATA3 and E2F3. (A) CCK-8 assays were performed to determine the ADR sensitivity of MCF-7/ADR cells cotransfected with sh-LOC645166 and pcDNA-NF-κB or pcDNA-GATA3. (B) CCK-8 assays were performed to determine the ADR sensitivity of 231 cells cotransfected with lnc-LOC645166 and si-NF-κB or si-GATA3. (C) Colony formation ability of 231/ADR cells was assessed when cotransfected with sh-LOC645166 and pcDNA-NF-κB or pcDNA-GATA3. (D) The apoptosis rate of 231/ADR cell cotransfected with sh-LOC645166 and pcDNA-NF-κB or pcDNA-GATA3 was measured by flow cytometric analysis. (E, F) Western blot assays were used to measure the expression of NF-κB, GATA3, c-PARP, c-Caspase-3 and p-STAT3 in 231/ADR and MCF-7/ADR cells cotransfected with sh-LOC645166 and pcDNA-NF-κB or pcDNA-GATA3. Data are shown as means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.