Figure 2. Girdin down-regulation regulates pancreatic cancer progression in vitro and in vivo. (A) Cell proliferation rates of both shCtrl and shGirdin cells were tested by CCK-8 assay. (B) APC/PI staining analysis to measure apoptosis levels of both shCtrl and shGirdin cells. Apoptosis levels were significantly higher in shGirdin cells. (C) Wound-healing assay was performed to examine the migration in PANC-1 and AsPC-1 cells with shCtrl or shGirdin transfection. (D) Transwell invasion assay was carried out in PANC-1 and AsPC-1 cells with shCtrl or shGirdin transfection. (E, F) Tumor growth curves were established by measuring tumor volume every 5 for 35 days after injection. Tumor weights isolated from nude mice in each treatment group were determined on day 28 after injection. Data was shown as mean ± SD. ***P<0.001. The experiments were repeated 3 times.