Figure 6. Cortactin protein level is decreased when knocking down Girdin. (A, B) Cortactin phosphorylation and acetylation. Cortactin was phosphorylated at S47 (A) and acetylated at K161 (B). (C) Expression of Girdin and Cortactin transcripts following shRNA knockdown of Girdin in PANC-1 cells was assessed by qPCR. (D) Western blotting results showed Girdin and Cortactin protein expression levels in shCtrl cells and shGirdin cells, with GAPDH loading control. Data was shown as mean ± SD. ***P<0.001. The experiments were repeated 3 times.