Figure 3. Effect of RES treatment on ROS levels in DXR-treated oocytes. (A) Representative images showed ROS levels in control, DXR-treated and RES-supplemented oocytes, Bar = 100 μm. (B) The fluorescence intensity of ROS was measured in control, DXR-treated and RES-supplemented oocytes. *means P < 0.05, **means P < 0.01. (C) Relative expressions of antioxidant genes Cat, Sod1, Sod2 and Gpx3 mRNA levels in oocytes. β-actin was used as a housekeeping gene. *means P < 0.05, **means P < 0.01. All experiments were performed in triplicates and the data were represented as the means ± SEM.