Figure 5. circRNA_141539 acts as a miRNA sponge for miR-4469. (A) Schematic diagram showed that miR-4469 specifically binds to 3′-UTR of circRNA_141539. (B) Dual-luciferase assay results. (C) qRT-PCR showed miR-4469 was were significantly down-regulated in ESCC tissues (n=50), *P < 0.05, **P < 0.01 versus corresponding adjacent tissues. (D) Pearson's correlation showed that circRNA_141539 was negatively correlated to miR-4469 expression. (E, F) qRT-PCR showed miR-4469 were significantly decreased in circRNA_141539 OE group, and dramatically increased in sh-circRNA_141539 group. *P < 0.05, **P < 0.01 versus corresponding NC group.