Research Paper Volume 13, Issue 8 pp 12179—12193

circRNA_141539 can serve as an oncogenic factor in esophageal squamous cell carcinoma by sponging miR-4469 and activating CDK3 gene

CDK3 was identified as a direct target of miR-4469. (A) Schematic diagram showed that miR-4469 specifically binds to the wild-type 3’-UTR sequence of CDK3. (B) Dual-luciferase assay results. (C) qRT-PCR results showed that CDK3 mRNA was significantly up-regulated in ESCC tissues (n=50), *P **P D) Pearson's correlation showed that CDK3 mRNA was negatively correlated to miR-4469 expression. (E) Western blot and (F) qRT-PCR results showed that the expression of CDK3 proteins and mRNA were significantly decreased in miR-4469 mimic group, and dramatically increased in miR-4469 inhibitor group. *P **P

Figure 6. CDK3 was identified as a direct target of miR-4469. (A) Schematic diagram showed that miR-4469 specifically binds to the wild-type 3’-UTR sequence of CDK3. (B) Dual-luciferase assay results. (C) qRT-PCR results showed that CDK3 mRNA was significantly up-regulated in ESCC tissues (n=50), *P < 0.05, **P < 0.01 versus corresponding adjacent tissues. (D) Pearson's correlation showed that CDK3 mRNA was negatively correlated to miR-4469 expression. (E) Western blot and (F) qRT-PCR results showed that the expression of CDK3 proteins and mRNA were significantly decreased in miR-4469 mimic group, and dramatically increased in miR-4469 inhibitor group. *P < 0.05, **P < 0.01 versus miR-4469 NC group.