Research Paper Volume 12, Issue 9 pp 7729—7746

Long noncoding RNA LINC00324 promotes retinoblastoma progression by acting as a competing endogenous RNA for microRNA-769-5p, thereby increasing STAT3 expression

Figure 2. LINC00324 knockdown inhibits Y79 and WERI-RB-1 cell proliferation, colony formation, migration, and invasion, and promotes apoptosis in vitro. (A) Either si-LINC00324 or si-NC was transfected into Y79 and WERI-RB-1 cells. The transfected cells were collected 48 h later and used for evaluation of transfection efficiency. *P < 0.05 vs. the si-NC group. (B, C) The proliferative and colony-forming abilities of LINC00324-depleted Y79 and WERI-RB-1 cells were examined using CCK-8 and colony formation assays, respectively. *P < 0.05 vs. group si-NC. (D, E) Apoptosis and cell cycle was analyzed by flow cytometry in Y79 and WERI-RB-1 cells transfected with either si-LINC00324 or si-NC. *P < 0.05 vs. group si-NC. (F, G) Transwell migration and invasion assays were performed to assess the migratory and invasive abilities of Y79 and WERI-RB-1 cells after transfection with either si-LINC00324 or si-NC. *P < 0.05 vs. the si-NC group.