Research Paper Volume 12, Issue 9 pp 7729—7746

Long noncoding RNA LINC00324 promotes retinoblastoma progression by acting as a competing endogenous RNA for microRNA-769-5p, thereby increasing STAT3 expression

Figure 4. Forced expression of miR-769-5p attenuates the growth and metastasis of Y79 and WERI-RB-1 cells in vitro. (A, B) Y79 and WERI-RB-1 cells were transfected with either agomir-769-5p or agomir-NC. After transfection, CCK-8 and colony formation assays were performed to assess the effects of miR-769-5p overexpression on the proliferative and colony-forming abilities of RB cells. *P < 0.05 vs. the agomir-NC group. (C, D) Apoptosis and cell cycle was assessed via flow-cytometric analysis in miR-769-5p–overexpressing Y79 and WERI-RB-1 cells. *P < 0.05 vs. group agomir-NC. (E, F) Y79 and WERI-RB-1 cells were treated as mentioned above, and Transwell migration and invasion assays were performed to assess cell migration and invasion. *P < 0.05 vs. group agomir-NC.