Research Paper Volume 12, Issue 9 pp 7729—7746

Long noncoding RNA LINC00324 promotes retinoblastoma progression by acting as a competing endogenous RNA for microRNA-769-5p, thereby increasing STAT3 expression

Figure 9. Depletion of LINC00324 attenuates the tumor growth of RB cells in vivo. (A) RT-qPCR analysis was utilized to assess the efficiency of sh-LINC00324 in Y79 cells. *P < 0.05 vs. the sh-NC. (B) The tumor growth rate was significantly suppressed by sh-LINC00324, as compared with that of the sh-NC group. *P < 0.05 vs. the sh-NC group. (C) At the conclusion of the assay, the mice were euthanized, and the tumor xenografts were excised. Representative images of tumor xenografts from the sh-LINC00324 and sh-NC groups. (D) The average weight of the tumor xenografts was determined in the sh-LINC00324 and sh-NC groups. *P < 0.05 vs. the sh-NC group. (E, F) RT-qPCR was carried out to analyze the expression of LINC00324 and miR-769-5p in the tumor xenografts. *P < 0.05 vs. group sh-NC. (G) Total protein was isolated from tumor xenografts, and then subjected to western blotting to measure STAT3 protein expression. *P < 0.05 vs. group sh-NC.