Research Paper Volume 12, Issue 13 pp 12684—12702

Silencing of long non-coding RNA Sox2ot inhibits oxidative stress and inflammation of vascular smooth muscle cells in abdominal aortic aneurysm via microRNA-145-mediated Egr1 inhibition

Figure 3. miR-145 suppresses the apoptosis, inflammatory reaction, and oxidative stress of VSMCs treated with Ang II. (A) the expression of the molecular marker α-SM-actin of VSMCs isolated by immunofluorescence assay (× 400); (B) miR-145 expression in VSMCs determined using RT-qPCR; (C) viability of VSMCs detected by CCK-8 assay; (D) apoptosis of VSMCs detected by flow cytometry; (E) levels of COX-2, NO, IL-1β, IL-6, and TNF-α in serum of VSMCs measured by ELISA; (F) levels of MDA and SOD in serum of VSMCs measured using kits; (G) O2- in VSMCs measured using DHE staining (× 400); (H) protein levels of cleaved caspase-3, NOX4, iNOS, p47phox, collagen I and collagen III in VSMCs determined using Western blot analysis; *, p < 0.05, vs. VSMCs without treatment; #, p < 0.05, vs. VSMCs treated with Ang II + LV-miR-NC or Ang II+LV-NC-inhibitor plasmids; measurement data were depicted as the mean ± standard deviation; comparisons among multiple groups were analyzed using one-way ANOVA followed by Turkey’s post hoc test; the experiment was repeated three times.