Research Paper Volume 12, Issue 13 pp 12684—12702

Silencing of long non-coding RNA Sox2ot inhibits oxidative stress and inflammation of vascular smooth muscle cells in abdominal aortic aneurysm via microRNA-145-mediated Egr1 inhibition

Figure 4. miR-145 is negatively regulated by lncRNA Sox2ot. (A) comparisons of predicted lncRNAs that target miR-145 in RAID database (http://www.rna-society.org/raid/index.html) and lncRNAs related to AAA reported in a prior study; (B) Sequence complementarity between lncRNA Sox2ot and miR-145 analyzed by RNA22; (C) lncRNA Sox2ot expression in abdominal aorta of normal mice and Ang II-induced AAA mice determined using RT-qPCR; (D) lncRNA Sox2ot expression in VSMCs determined by RT-qPCR; (E) miR-145 expression in VSMCs measured by RT-qPCR; (F) RIP assay with anti-Ago2, IgG, or 10% input from VSMC extracts. RNA levels in the immunoprecipitates were determined by RT-qPCR; (G) co-localization of miR-145 and lncRNA Sox2ot detected by FISH (× 400); * p < 0.05, vs. VSMCs without treatment; # p < 0.05, vs. VSMCs treated with Ang II + LV-miR-NC or Ang II + LV-NC-inhibitor plasmids; measurement data were depicted as the mean ± standard deviation; comparisons between the two groups were analyzed using an unpaired t-test, and comparisons among multiple groups were analyzed using one-way ANOVA, followed by Turkey’s post hoc test; each experiment was repeated three times.