Research Paper Volume 12, Issue 13 pp 12684—12702

Silencing of long non-coding RNA Sox2ot inhibits oxidative stress and inflammation of vascular smooth muscle cells in abdominal aortic aneurysm via microRNA-145-mediated Egr1 inhibition

Figure 5. LncRNA Sox2ot reverses the inhibitory roles of miR-145 in oxidative stress and inflammation of VSMCs treated with Ang II. (A) viability of VSMCs detected by CCK-8 assay; (B) apoptosis of VSMCs detected by flow cytometry; (C) levels of COX-2, NO, IL-1β, IL-6, and TNF-α in serum of VSMCs measured by ELISA; (D) levels of MDA and SOD in serum of VSMCs measured using kits; (E) ROS level in VSMCs measured using kits; (F) protein levels of cleaved caspase-3, NOX4, iNOS, p47phox, collagen I and collagen III determined using Western blot analysis; * p < 0.05, vs. VSMCs without treatment; # p < 0.05, vs. VSMCs treated with Ang II + NC-mimic, Ang II + NC-inhibitor, and Ang II + miR-145-mimic + NC plasmids; measurement data were depicted as the mean ± standard deviation; comparisons among multiple groups were analyzed using one-way ANOVA followed by Turkey’s post hoc test; the experiment was repeated three times.