Research Paper Volume 12, Issue 13 pp 12684—12702

Silencing of long non-coding RNA Sox2ot inhibits oxidative stress and inflammation of vascular smooth muscle cells in abdominal aortic aneurysm via microRNA-145-mediated Egr1 inhibition

Figure 7. Silencing of lncRNA Sox2ot affecting miR-145 inhibits oxidative stress and inflammation in Ang II-induced AAA mice by downregulating Egr1. (A) representative morphology images of abdominal aorta specimens from mice; (B) incidence of inducing AAA mice; (C) the maximum diameter of abdominal aorta from mice; (D) α-SM-actin expression in SMCs in abdominal aorta determined using immunohistochemistry (× 400); (E) CD68 expression in abdominal aorta detected using immunofluorescence staining (× 400); (F) levels of inflammatory factors and oxidative stress-related factors measured in serum of mice measured using ELISA; (G) SOD level in serum and MDA levels in abdominal aorta of mice; (H) protein levels of Egr1, M-CSF, MCP-1, MIP-2, ICAM-1, cleaved caspase-3, iNOS, p47phox, collagen I and collagen III determined using Western blot analysis; * p < 0.05, vs. normal mice; # p < 0.05, vs. AAA mice injected with LV-NC; & p < 0.05, vs. AAA mice injected with LV-Sox2ot; @ vs. AAA mice, injected with LV-Egr1; measurement data were depicted as the mean ± standard deviation; comparisons among multiple groups were analyzed using one-way ANOVA followed by Turkey’s post hoc test; n = 10.