Figure 1. Melatonin treatment restores mitochondrial biogenesis in H/R-treated cardiomyocytes. Cardiomyocytes were subjected to H/R injury, with or without previous melatonin treatment to protect the cardiomyocytes. (A–D) qPCR assays were used to evaluate the transcription of PGC1α, Tfam, Nrf2 and Sirt3. (E, F) Western blots were used to evaluate the alterations in the mitochondria-related proteins Tom20 and Tim23. *p<0.05.