Figure 2. Melatonin activates the AMPK pathway to induce mitochondrial biogenesis. Cardiomyocytes were subjected to H/R injury, with or without previous melatonin treatment to protect the cardiomyocytes. (A) An ELISA was used to evaluate AMPK activity. (B, C) An immunofluorescence assay was used to evaluate the expression of p-AMPK. (D, E) qPCR was used to evaluate the transcription of Tfam and Nrf2. CC was used to inhibit the activity of AMPK. *p<0.05.