Research Paper Volume 12, Issue 8 pp 7299—7312

Melatonin improves mitochondrial biogenesis through the AMPK/PGC1α pathway to attenuate ischemia/reperfusion-induced myocardial damage

Figure 2. Melatonin activates the AMPK pathway to induce mitochondrial biogenesis. Cardiomyocytes were subjected to H/R injury, with or without previous melatonin treatment to protect the cardiomyocytes. (A) An ELISA was used to evaluate AMPK activity. (B, C) An immunofluorescence assay was used to evaluate the expression of p-AMPK. (D, E) qPCR was used to evaluate the transcription of Tfam and Nrf2. CC was used to inhibit the activity of AMPK. *p<0.05.