Figure 3. Melatonin preserves mitochondrial function in H/R-treated cardiomyocytes by inducing the AMPK/PGC1α pathway. Cardiomyocytes were subjected to H/R injury, with or without previous melatonin treatment to protect the cardiomyocytes. The cardiomyocytes were transfected with siRNA to knock down PGC1α. (A) An ELISA was used to assess ATP production. (B, C) An immunofluorescence assay was used to measure mitochondrial ROS in cardiomyocytes. (D, E) An immunofluorescence assay was used to measure the mitochondrial membrane potential. *p<0.05.