Research Paper Volume 12, Issue 8 pp 7299—7312

Melatonin improves mitochondrial biogenesis through the AMPK/PGC1α pathway to attenuate ischemia/reperfusion-induced myocardial damage

Figure 4. Melatonin alters the mitochondrial morphology in cardiomyocytes by inducing the AMPK/PGC1α pathway. Cardiomyocytes were subjected to H/R injury, with or without previous melatonin treatment to protect the cardiomyocytes. The cardiomyocytes were transfected with siRNA to knock down PGC1α. (A, B) An immunofluorescence assay was used to assess the mitochondrial morphology. (C, D) A qPCR assay was used to evaluate the transcription of Opa1 and Mfn2. (E, F) An mt-kemia assay was used to evaluate mitophagy activity. *p<0.05.