Research Paper Volume 12, Issue 10 pp 8913—8922

HIX003209 promotes vascular smooth muscle cell migration and proliferation through modulating miR-6089

Figure 3. Ectopic expression of HIX003209 induced inflammatory mediators secretion, cell growth and migration in VSMCs. (A) The expression of HIX003209 was measured with qRT-PCR assay. (B) Ectopic expression of HIX003209 induced inflammatory mediators secretion including IL-6 compared to control group. (C) The expression of TNF-α in cell culture supernatant was measured by ELISA assay. (D) The expression of IL-1β in cell culture supernatant was measured by ELISA assay. (E) Elevated expression of HIX003209 promoted VSMCs proliferation compared to control group. (F) The expression of cyclin D1 was measured by qRT-PCR analysis. (G) Overexpression of HIX003209 induced VSMCs migration compared to control group. (H) The relative wound breadth remain of two groups was showed **p<0.01 and ***p<0.001. GAPDH was used as internal control.