Research Paper Volume 12, Issue 9 pp 7761—7773

MicroRNA-1298-3p inhibits proliferation and invasion of glioma cells by downregulating Nidogen-1

Figure 4. Overexpression of miR-1298-3p induces apoptosis of glioma cells. Apoptosis analyzed by Annexin V and PI double staining in (A, B) U87MG and (C, D) SHG44 cells transfected with miR-1298-3p agonist for 72 h. (E) Western analysis of Bax, active caspase 3, and Bcl-2 levels in U87MG and SHG44 cells. (FH) The relative expression of Bax, active caspase 3, and Bcl-2 in U87MG and SHG44 cells, normalized to β-actin; **P < 0.01 compared with the NC group.