Research Paper Volume 12, Issue 9 pp 7761—7773

MicroRNA-1298-3p inhibits proliferation and invasion of glioma cells by downregulating Nidogen-1

Figure 7. Upregulation of miR-1298-3p inhibits tumorigenesis of U87MG subcutaneous xenografts in vivo. U87MG cells were subcutaneously injected into nude mice, and 50 nM miR-1298-3p agonist was directly injected into the tumors twice a week. (A) Xenograft tumor volume was monitored weekly. (B, C) Xenografts tumors were photographed and calculated. (D, E) TUNEL assay of apoptosis in tumor tissues. (F, G) IHC assay of proliferation in tumor tissues. (H) Protein levels of NID1, E-cadherin, and vimentin in tumor tissues analyzed by western blotting. (IK) The relative expression of NID1, E-cadherin, and vimentin in tumor tissues quantified, and normalized to β-actin. (L) The body weights of mice were monitored. **P < 0.01 compared with the NC group.