Research Paper Volume 12, Issue 9 pp 7774—7785

Hsa_circ_0018818 knockdown suppresses tumorigenesis in non-small cell lung cancer by sponging miR-767-3p

Figure 5. MiR-767-3p is the downstream target of hsa_circ_0018818. (A, B) Gene structure of hsa_circ_0018818 indicating the predicted miR-767-3p binding site in its 3'UTR. (C) RT-qPCR analysis miR-767-3p expression in NCI-H1650 cells. (D) The luciferase activity in NCI-H1650 cells after co-transfecting a plasmid encoding the wild-type (WT) or mutant (MT) hsa_circ_0018818 3′-UTR and miR-767-3p. (E) Co-localization of hsa_circ_0018818 and miR-767-3p detected using FISH. **P< 0.01 vs. control. (F) Gene structure of NID1 at the position of bp 161-167 showing the predicted miR-767-3p binding site in its 3'UTR. (G) Luciferase activity in NCI-H1650 cells after co-transfecting a plasmid encoding the WT or MT NID1 3′-UTR and miR-767-3p. **P< 0.01 vs. control.