Figure 8. Hsa_circ_0018818 shRNA1 suppresses NSCLC tumor growth in vivo. Mice were subcutaneously injected NC1-H1650 transfected with vector-control or hsa_circ_0018818 shRNA1 or left untreated (Blank), after which tumors were allowed to grow for 4 weeks. (A) Volumes of tumors collected at the indicted times after transplantation. (B) Images of tumors (left) and tumor weights (right) after 4 weeks. (C) RT-qPCR analysis of hsa_circ_0018818 gene expression in tumor tissues. (D) Western blot analysis of NID1, Akt, ERK, p-Akt and p-ERK levels in tumor tissues. (E–G) Relative levels of NID1, p-Akt and p-ERK expression normalized to β-actin expression. **P< 0.01 vs. control.