Figure 4. The permeabilization effects of hAmylin on neurons and astrocytes. (A, B) Neuron-specific fluorescence (MAP2) was observed in neurons after their incubation with Triton X-100. When Triton X-100 was replaced with PBS, intracellular fluorescence was almost invisible. However, when 10 μM hAmylin was used instead of Triton X-100 (incubation for 1 min or 30 min), intracellular fluorescence could still be observed in neurons. (C) The percentage of positive cells was 31.01% for 1 min incubation and 35.15% for 30 min incubation. (D–F) In contrast, astrocyte-specific fluorescence (S100) was not clearly observed when 10 μM hAmylin was used instead of Triton X-100 (incubation for 1 min). However, when the incubation time was prolonged to 30 min, intracellular fluorescence could be observed (D, E) in 34.65% of astrocytes (F). ***p < 0.001 versus PBS group (chi-square test).