Research Paper Volume 12, Issue 10 pp 8923—8938

Influence of human amylin on the membrane stability of rat primary hippocampal neurons

Figure 4. The permeabilization effects of hAmylin on neurons and astrocytes. (A, B) Neuron-specific fluorescence (MAP2) was observed in neurons after their incubation with Triton X-100. When Triton X-100 was replaced with PBS, intracellular fluorescence was almost invisible. However, when 10 μM hAmylin was used instead of Triton X-100 (incubation for 1 min or 30 min), intracellular fluorescence could still be observed in neurons. (C) The percentage of positive cells was 31.01% for 1 min incubation and 35.15% for 30 min incubation. (DF) In contrast, astrocyte-specific fluorescence (S100) was not clearly observed when 10 μM hAmylin was used instead of Triton X-100 (incubation for 1 min). However, when the incubation time was prolonged to 30 min, intracellular fluorescence could be observed (D, E) in 34.65% of astrocytes (F). ***p < 0.001 versus PBS group (chi-square test).