Research Paper Volume 12, Issue 10 pp 8939—8952

miR-378a-3p inhibits ischemia/reperfusion-induced apoptosis in H9C2 cardiomyocytes by targeting TRIM55 via the DUSP1-JNK1/2 signaling pathway

Figure 1. miR-378a-3p is upregulated in I/R-induced H9C2 cardiomyocytes and inhibits cell apoptosis. (A) miR-378a-3p expression was measured by Real-time PCR in H9C2 cardiomyocytes following 3 h ischemia and 6, 12 or 24 h reperfusion. H9C2 cardiomyocytes following 3 h ischemia and 24 h reperfusion were transfected with a miR-378a-3p mimic, inhibitor or negative control (NC) and those without I/R injury were used as a control. (B) miR-378a-3p expression, (C, D) cell apoptosis, and (EJ) protein expression of DUSP1, p-JNK1/2, JNK1/2, cleaved PARP and caspase-3, Bax, and Bcl-2 were measured. ***P < 0.001 compared with 0 h or I/R. #P < 0.05, ##P < 0.01, ###P<0.001 compared with I/R + NC.