Research Paper Volume 12, Issue 10 pp 8939—8952

miR-378a-3p inhibits ischemia/reperfusion-induced apoptosis in H9C2 cardiomyocytes by targeting TRIM55 via the DUSP1-JNK1/2 signaling pathway

Figure 3. TRIM55 silencing inhibits I/R- and miR-378a-3p inhibitor-induced apoptosis of H9C2 cardiomyocytes. H9C2 cardiomyocytes were transfected with three TRIM55-siRNAs (siRNA-1, siRNA-2, siRNA-3) or scramble siRNA (siNC). (A, B) TRIM55 expression was measured. H9C2 cardiomyocytes following I/R injury were transfected with the TRIM55-siRNA and/or miR-378a-3p inhibitor. (C, D) Cell apoptosis was measured by flow cytometry. (EH) Expression of TRIM55, DUSP1, JNK1/2, cleaved PARP and caspase-3, Bax, and Bcl-2 was measured. ***P < 0.001 compared with siNC or I/R + NC + siRNA. ###P < 0.001 compared with I/R + inhibitor.