Figure 5. DUSP1 overexpression inhibits I/R- and TRIM55 overexpression-induced apoptosis of H9C2 cardiomyocytes. H9C2 cardiomyocytes following I/R injury were transduced with a TRIM55 and/or DUSP1 expression vector or blank vector. (A, B) Cell apoptosis was measured by flow cytometry. (C–H) The expression of DUSP1, JNK1/2, JNK1/2, cleavage of PARP and caspase-3, Bax, and Bcl-2 was also measured. ***P < 0.001 compared with I/R + vector. ###P < 0.001 compared with I/R + TRIM55.