Research Paper Volume 12, Issue 10 pp 8939—8952

miR-378a-3p inhibits ischemia/reperfusion-induced apoptosis in H9C2 cardiomyocytes by targeting TRIM55 via the DUSP1-JNK1/2 signaling pathway

Figure 6. The miR-378a-3p mimic inhibits I/R-induced apoptosis in rats. Myocardial I/R model rats were injected with 50 mg/kg of the miR-378a-3p mimic or negative control (NC) 24 h before LCA ligation. (AC) Histological assessment of the myocardium was performed by H&E staining and TUNEL. Scale bar: 100 μm. (DF) The expression of miR-378a-3p, TRIM55, Bax, and Bcl-2 was measured by real-time PCR. (G) The expression of TRIM55, DUSP1, p-JNK1/2, JNK1/2, cleavage of PARP and caspase-3, Bax, and Bcl-2 was measured by western blotting. ***P < 0.001 compared with sham. #P < 0.05, ##P < 0.01, ###P < 0.001 compared with I/R + NC. ΔP < 0.05, ΔΔΔP < 0.001 compared with I/R + mimic.