Figure 2. CCI induced astrocytic P2Y1R up-regulation and JNK activation in the spinal cord. (A) Immunostaining showing that CCI injury induced astrocytic marker GFAP expression in the ipsilateral spinal cord on POD 14. JNK phosphorylation and P2Y1R up-regulation occurred accompanied with astrocyte activation. Scale bar=200μm. (B) Western blot showing that GFAP, p-JNK and P2Y1R were increased in CCI group compared with sham group. All data are means ± S.D. ###p<0.01, compared with sham group; n=3 mice/group. The differences between groups were analyzed by ANOVA followed by Bonferroni post-hoc test.