Figure 8. Osthole exerts analgesic effect by directly acting on P2Y1 receptor of astrocyte. (A) P2Y1R expression in transfected cells markedly increase compared to normal astrocyte. (B) Astrocyte-conditioned medium induced the change trend of c-Fos consistent with that of P2Y1R. ###p<0.01, compared with sham group; ***p<0.01, compared with P2Y1R overexpression group, ANOVA followed by Bonferroni post-hoc test, n=6 mice/group. All data are means ± S.D.